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Image Search Results


The exosomal protein NID1 promotes liver metastasis of CRC tumors (A) EV mouse model comparing the effects of EVs from SW480/km12-LM3 and shNID1-1 SW480/km12-LM3 cells on CRC metastasis ( n = 5). (B and C) Image showing the luciferase signal of the animals at the end of the experiment. Quantification of the luciferase signal is shown. (D) Comparison of the effects of EVs from XPack and XP-NID1 HCT116 cells on CRC metastasis in an EV mouse model ( n = 5). (E and F) Image showing the luciferase signal of the animals at the end of the experiment. Quantification of the luciferase signal is shown. (G) Schematic diagram of the mouse model of in situ cecum injection combined with splenic injection. (H and I) In vivo imaging images of the mice and quantitative fluorescence statistics of the mouse liver region. n.s. represents not significant; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.001, analyzed with a t test (B, C, E, F, H, and I). Data are represented as mean ± SEM.

Journal: iScience

Article Title: Nidogen 1-enriched extracellular vesicles promote liver metastasis by inducing EMT and activating stellate cells

doi: 10.1016/j.isci.2025.113975

Figure Lengend Snippet: The exosomal protein NID1 promotes liver metastasis of CRC tumors (A) EV mouse model comparing the effects of EVs from SW480/km12-LM3 and shNID1-1 SW480/km12-LM3 cells on CRC metastasis ( n = 5). (B and C) Image showing the luciferase signal of the animals at the end of the experiment. Quantification of the luciferase signal is shown. (D) Comparison of the effects of EVs from XPack and XP-NID1 HCT116 cells on CRC metastasis in an EV mouse model ( n = 5). (E and F) Image showing the luciferase signal of the animals at the end of the experiment. Quantification of the luciferase signal is shown. (G) Schematic diagram of the mouse model of in situ cecum injection combined with splenic injection. (H and I) In vivo imaging images of the mice and quantitative fluorescence statistics of the mouse liver region. n.s. represents not significant; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.001, analyzed with a t test (B, C, E, F, H, and I). Data are represented as mean ± SEM.

Article Snippet: NID1 was expressed through XPack EV Protein Engineering Technology (System Biosciences) for expression in the EVs of HCT116 and NCM-460 cells.

Techniques: Luciferase, Comparison, In Situ, Injection, In Vivo Imaging, Fluorescence